Analyses of fungal fermentation of lignocellulosic substrates using continuous culture rumen simulation.

Continuous Culture as a Method for Studying Rumen Fermentation.

Effect of the magnitude of the decrease of rumen pH on rumen fermentation in a dual-flow continuous culture system.

The negative effect of pH on rumen microbial fermentation has been associated with the total amount of time that pH is below a certain threshold. However, not only the time, but also the magnitude of the pH reduction, is important. Eight 1,325-mL dual-flow continuous culture fermenters were used in 2 replicated periods to determine the effect of the magnitude of pH reduction (5.6 vs. 5.1) durin...

Effects of dry plant extracts on fermentation and methanogenesis in continuous culture of rumen microbes

Thirteen plant extracts, selected for their high ̄avonoid content, were screened on their action on fermentation and protozoa numbers in 1 l dual out ̄ow fermenters supplied with a 50:50 orchard grass hay‡ barley diet. Using a 214ÿ10 fractional factorial design, we tested each extract at the rate of 0.5 g per day. The corresponding organic matter in ̄ow was equal to 3.4% of the basal hexoses ferm...

Effect of different levels of phosphorus on rumen microbial fermentation and synthesis determined using a continuous culture technique.

A continuous culture technique was used to study the phosphorus requirements of rumen micro-organisms. Solutions of artificial saliva containing 120, 80, 40 and 0 mg inorganic phosphorus (Pi)/l were infused into the reaction vessels previously inoculated with rumen contents, resulting in Pi concentrations in the vessel contents of 48, 28, 4 and less than 1 mg/l respectively. Various fermentativ...

Effect of Protein Sources on Rumen Microbial Protein Synthesis Using Rumen Simulated Continuous Culture System

A rumen simulated continuous culture (RSCC) system was used to study the influence of supplementation of the three different types of protein sources such as urea, casein and soy protein on rumen microbial synthesis in terms of rumen microbial synchronization. The urea treatment showed the highest pH value. Ammonia nitrogen concentration was rapidly increased after feeding and not significantly...